作者：袁欣¹, 苟在旭², 郭琦², 周玉², 贺字英², 方玉宇², 江雪², 黄科²

作者单位：1. 成都中医药大学药学院, 四川 成都 611137;
2. 四川师范大学化学与材料科学学院, 四川 成都 610068

关键词：四氧化三铁磁性纳米材料;氢化物发生;砷;原子荧光;富集

摘要：

该文提出一种基于四氧化三铁磁性纳米材料（Fe3O4 MNPs）分离富集细胞及环境样品中砷的新方法。Fe3O4 MNPs可快速吸附细胞溶液中的无机砷，并利用其磁性可在磁场作用下与基体溶液直接分离，从而实现对砷的高效富集。向分离后的Fe3O4 MNPs中加入少量盐酸使其溶解，即可通过氢化物发生原子荧光光谱法准确测定砷含量。该方法的检出限为0.004 ng/mL，相当于未富集时的1/50。方法的相对标准偏差（RSD）小于2.0%（n=7），表明其具有较高的稳定性。该方法具有操作简单、抗干扰能力强、灵敏度高、绿色环保等优点。可成功用于细胞样品中砷的含量测定，分析结果令人满意。

Study on determination of arsenic by hydride generation-atomic fluorescence spectrometry using Fe₃O₄ magnetic nanoparticles for preconcentration

YUAN Xin¹, GOU Zaixu², GUO Qi², ZHOU Yu², HE Ziying², FANG Yuyu², JIANG Xue², HUANG Ke²

1. College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China;
2. College of Chemistry and Materials Science, Sichuan Normal University, Chengdu 610068, China

Abstract: In this work, a novel method was developed by using Fe₃O₄ magnetic nanoparticles (MNPs) for effective adsorption of inorganic arsenic (As(Ⅲ)) in environmental samples and cells for preconcentration. The adsorbed inorganic arsenic by Fe₃O₄ MNPs in cells was easily separated by a magnet from the sample matrix. And the Fe₃O₄ MNPs was completely dissolved in hydrochloric acid prior to the determination of inorganic arsenic by hydride generation-atomic fluorescence spectrometry (HG-AFS). The limit of detection (LOD) was 0.004 ng/mL for As(Ⅲ), which was improved by 50-fold compared to that of conventional method without preconcentration. The relative standard deviation (RSD) was less than 2.0% (n=7),with high stability. The method has the advantages of simple operation, strong anti-interference ability, high sensitivity and green environmental protection, which can be used successfully for the determination of arsenic in cell samples with satisfactory results

Keywords: Fe₃O₄ MNPs;hydride generation;As(Ⅲ);atomic fluorescence spectrometry;preconcentration

2018, 44(9): 51-56  收稿日期: 2018-02-07;收到修改稿日期: 2018-03-16

基金项目: 国家自然科学基金（81603291）；成都中医药大学校基金（ZRQN1651）

作者简介: 袁欣(1988-),女,四川成都市人,讲师,博士,主要从事光谱分析方面研究

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