作者：王伟玲, 王晓玲
作者单位：西南民族大学化学与环境保护工程学院, 四川 成都 610041
关键词：大马士革玫瑰花;精油;气相色谱-质谱联用（GC-MS）;分离鉴定
摘要：
以贵州黔南平塘县、陕西三原县两个地区种植的大马士革玫瑰花为材料，建立玫瑰花精油提取分离以及主要挥发性成分的鉴定方法，通过改进分析方法，比较不同地区种植的大马士革玫瑰花精油的主要香气成分。利用水蒸气蒸馏法提取玫瑰花精油，应用气相色谱-质谱联用（GC-MS）法对提取的玫瑰花精油成分进行定性分离鉴定。共鉴定28种主要化合物，与国际标准中玫瑰精油的组成成分及相对含量范围基本一致。所测的几种化学成分精密度、重复性和稳定性良好；加标回收率为97.1%~99.4%，RSD为0.80%~1.50%。不同产地的大马士革玫瑰花精油的主要成分和相对含量有一定差异，各具特色。本实验建立的分析方法稳定，适合玫瑰花精油的成分分析。
Research on chemical components of damascus Rose essential oil from different areas
WANG Weiling, WANG Xiaoling
College of Chemistry & Environment Protection Engineering, Southwest Minzu University, Chengdu 610041, China
Abstract: To establish a method for extracting and separating rose essential oil and the separation and identification of main volatile components from damascus Rose a planted in Pingtang County of Guizhou Province and Sanyuan County of Shaanxi Province, the analytical methods were improved to compare the main aroma components of damascus Rose essential oil grown in different regions by improving the analytical methods. Rose essential oil was extracted by steam distillation, and the extracted rose essential oil components were qualitatively separated and identified by gas chromatography-mass spectrometry (GC-MS). A total of 28 major compounds were identified, which are basically consistent with the composition and relative content of rose essential oils in international standards. The precision, repeatability and stability of several chemical components tested were great; the recoveries were 97.1%-99.4%, and the RSD was 0.80%-1.50%. The main components and relative contents of damascus Rose essential oils from different regions have different characteristics and different characteristics. The analytical method established in this experiment is stable and suitable for the composition analysis of rose essential oil.
Keywords: damascus Rose;essential oil;gas chromatography-mass spectrometry (GC-MS);separation and identification
2019, 45(3):59-64 收稿日期: 2018-11-01;收到修改稿日期: 2018-12-13
基金项目: 四川省科技支撑计划项目（2015SZ0040）；西南民族大学研究生创新型科研项目（CX2018SZ142）
作者简介: 王伟玲(1984-),女,内蒙古通辽市人,硕士,主要从事天然产物的分离分析
参考文献
[1] 张媛媛. 水蒸气蒸馏提取玫瑰精油的工艺研究[J]. 吉林农业, 2016(20):119
[2] 李玉杰, 刘晓蕾, 刘霞, 等. 玫瑰精油的化学成分及其抗菌活性[J]. 植物研究, 2009, 29(4):488-491
[3] 厍文波, 甘露, 安晓晶, 等. 玫瑰精油抗血栓形成作用[J]. 食品科学, 2011, 32(11):270-272
[4] 王金翠, 杨森艳, 李树发, 等. 拉萨曲水地区引种大马士革玫瑰的精油得率及成分研究[J]. 香料香精化妆品, 2015(5):11-16
[5] 陈秋鹏, 黄荣萍, 李雪菲, 等. 新疆和田地区大马士革玫瑰精油GC/MS指纹图谱分析[J]. 新疆中医药, 2015, 33(5):55-58
[6] 杨柳, 崔亚宁, 刘松, 等. 大马士革玫瑰1号精油香气成分的分析[J]. 北京农学院学报, 2015, 30(3):19-23
[7] 郭永来, 吕传润, 赵杰, 等. 利用分子蒸馏技术提取玫瑰油的工艺研究[J]. 香料香精化妆品, 2010(4):11-13
[8] 薛晓丽. 超临界CO2萃取玫瑰精油的优化工艺研究[J]. 江苏农业科学, 2010(2):307-309
[9] 牛晓娜, 刘宁, 莫世彬, 等. 植物精油的新型提取工艺及其研究进展[J]. 中国洗涤用品工业, 2013(4):80-82
[10] DOBREVA A, VELCHEVA A, BARDAROV V, et al. Chemical composition of different genotypes oil bearing roses[J]. Bulgarian Journal of Agricultural Science, 2013, 19(6):1213-1218
[11] 张睿, 魏安智, 杨途熙, 等. 商州产大马士革玫瑰精油研究[J]. 西北植物学报, 2005(7):1477-1479
[12] 余珍, 易元芬, 吴玉, 等. 几种玫瑰油的化学成分及香气比较[J]. 云南植物研究, 1994(1):75-80
[13] 李国明, 张丽萍, 刘小琼, 等. 香槟玫瑰花挥发油化学成分分析[J]. 安徽农业科学, 2018, 46(10):159-161+211
[14] 徐易. 玫瑰油国际标准(ISO9842:2003)[J]. 香料香精化妆品, 2003(6):36-37
