作者：韩雨桐, 栾荣生

作者单位：四川大学华西公共卫生学院/四川大学华西第四医院，四川 成都 610041

关键词：新型冠状病毒;新型冠状病毒肺炎;核酸检测;诊断

摘要：

自新型冠状病毒肺炎爆发以来，疫情迅速蔓延至全国各地区。准确并及时诊断新型冠状病毒感染对患者的治疗以及疫情的防控至关重要。基于实时荧光RT-PCR技术的病毒核酸检测是新型冠状病毒肺炎诊断的主要手段。该文简要介绍新型冠状病毒核酸检测技术的原理及流程，并从疾病特点、检测试剂盒的性能、临床采样过程、实验操作等方面分析可能影响检测结果准确性的因素，为进一步提高新型冠状病毒核酸检测效能，降低漏诊率提供依据。

Analysis of the application of nucleic acid testing in the diagnosis for novel coronavirus pneumonia
HAN Yutong, LUAN Rongsheng
West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, China
Abstract: Since the outbreak of the new coronavirus pneumonia , the epidemic has spread rapidly nationwide. Accurate and timely diagnosis of the infection of 2019-nCoV is crucial to the treatment of patients and the prevention and control of the epidemic. The nucleic acid testing of RT-PCR is the main method for the diagnosis of novel coronavirus pneumonia. This paper briefly introduces the principle and process of nucleic acid testing for 2019-nCoV, and analyzes the factors that may affect the accuracy of the detection results in terms of disease characteristics, detection kit performance, clinical sampling, experimental operation, etc., so as to provide bases for effectively improving the detection efficiency, especially reducing rate of missed diagnosis of the nucleic acid testing for 2019-nCoV.
Keywords: 2019-nCoV;novel coronavirus pneumonia;nucleic acid testing;diagnosis
2020, 46(3):73-76  收稿日期: 2020-02-18;收到修改稿日期: 2020-03-10
基金项目:
作者简介: 韩雨桐(1996-),女,黑龙江鹤岗市人,硕士研究生,专业方向为行为流行病学
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