微波辅助萃取-高效液相色谱法同时测定PM2.5中16种多环芳烃
作者:刘滔, 游钒, 袁小雪, 谢碧俊, 雍莉, 胡彬 作者单位:四川省疾病预防控制中心理化检验所, 四川 成都 610041 发布时间:2018-07-02 09:30:11 点击数:119

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关键词:微波萃取;PM2.5;多环芳烃;高效液相色谱法
摘要:

建立磁力搅拌辅助微波萃取-高效液相色谱法同时测定PM2.5中16种多环芳烃(PAHs)的方法。以玻璃纤维滤膜采样,滤膜经正己烷-丙酮(v/v 2:1)于磁力搅拌微波萃取仪中提取,提取液经浓缩后用乙腈定容,以乙腈和水作为流动相,通过高效液相色谱反相梯度洗脱分离,紫外和荧光串联检测器检测。16种PAHs的线性范围为0.025~5 μg/mL,平均回收率为78.8%~101.6%,相对标准偏差为0.4%~5.8%,检出限为0.005~0.051 ng/m3。该法快速、准确、灵敏、溶剂用量小,满足PM2.5中多环芳烃的痕量分析要求。

Simultaneous determination of 16 polycyclic aromatic hydrocarbons in PM2.5 by microwave-assisted extraction coupled with high performance liquid chromatography

LIU Tao, YOU Fan, YUAN Xiaoxue, XIE Bijun, YONG Li, HU Bin

Institute of Physicochemical Detection, Sichuan Center for Disease Control and Prevention, Chengdu 610041, China

Abstract: A method for the simultaneous determination of polycyclic aromatic hydrocarbons (PAHs) in PM2.5 using magnetic stirring-microwave extraction coupled with high performance liquid chromatography was developed. Atmospheric particulates were collected by glass fiber membrane filter. The glass fiber membrane filter was extracted by adding n-hexane/acetone(v/v 2:1) in an magnetic stirring-microwave extraction apparatus. Extracting solution was added with acetonitrile to form a constant volume after enrichment, and separated by reversed phase HPLC and gradient elution with acetonitrile and water as mobile phase, then detected by ultraviolet-fluorescence detector. The linear range of 16 PAHs ranged from 0.025 μg/mL to 5 μg/mL. The average recoveries were from 78.8% to 101.6%. The relative standard deviations were between 0.4% and 5.8%. The detection limits ranged from 0.005 ng/m3 to 0.051 ng/m3. The method is rapid, accurate, sensitive, solvent saved, and it meets the trace analysis requirements of PAHs in PM2.5.

Keywords: microwave extraction;PM2.5;polycyclic aromatic hydrocarbons;high performance liquid chromatography

2018, 44(6): 48-53  收稿日期: 2017-08-23;收到修改稿日期: 2017-10-25

基金项目: 

作者简介: 刘滔(1988-),男,四川资阳市人,主管技师,硕士,研究方向为卫生理化检验。

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