作者：赵兴春1, 孙敬1, 印佳2, 王燕2, 姜伯玮3, 高运华4, 叶健1
作者单位：1. 公安部物证鉴定中心, 北京 100038;
2. 中国人民公安大学, 北京 100038;
3. 中科院应用物理研究所, 上海 201800;
4. 中国计量科学研究院, 北京 100013
关键词：法医DNA; 标准物质; STR位点; 等位基因; 定值
摘要：
为探讨法医DNA标准物质STR位点等位基因分型的定值和溯源,利用有机法提取法医DNA标准物质备选所用细胞基因组DNA,以其为模板进行STR复合扩增,制备获得等位基因分型片段并测序分析,成功获得法医DNA标准物质备选所用细胞STR位点等位基因分型的定值。对STR位点等位基因分型进行定值研究可以作为法医DNA标准物质溯源研究的有效途径之一。
Certified value study for alleles on STR loci of forensic DNA reference materials
ZHAO Xing-chun1, SUN Jing1, YIN Jia2, WANG Yan2, JIANG Bo-wei3, GAO Yun-hua4, YE Jian1
1. Institute of Forensic Science, Ministry of Public Security, Beijing 100038, China;
2. Chinese People's Public Security University, Beijing 100038, China;
3. Applied Physics Institute, Chinese Academy of Science, Shanghai 201800, China;
4. National Institute of Metrology, Beijing 100013, China
Abstract: In order to develop the certified value and traceability for the alleles on STR loci of forensic DNA reference materials, the genomic DNA of the cells was extracted and amplified for preparing the forensic DNA reference materials. Then the allelic DNA was prepared, sequenced and valuated. Finally the value for the alleles on STR loci of the cells was certified. The certified value for the alleles on STR loci can work as an effective approach for the traceability of forensic DNA reference materials.
Keywords: forensic DNA; reference materials; STR loci; alleles; certified value
2012, 38(4): 55-57 收稿日期: 2011-9-15;收到修改稿日期: 2011-11-27
基金项目: 国家"十一五"科技支撑计划项目(2008BAK45B04)
作者简介: 赵兴春(1974-),男,湖南常德市人,副主任法医师,主要从事法庭科学DNA检验和相关技术研究。
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