作者：高宇1, 廖彩羽1, 汤科1, 程道梅1, 赵长菘1, 刘鑫1, 张弛1, 韩云蕾2
作者单位：1. 成都医学院公共卫生学院, 四川 成都 610500;
2. 成都医学院基础医学院, 四川 成都 610500
关键词：降胆固醇;乳酸菌;泡菜;益生菌;筛选
摘要：
为筛选出具有降胆固醇能力且益生性能优良的乳酸菌，从28份四川传统泡菜盐水中分离得到103株乳酸菌。通过体外胆固醇降解能力、耐酸耐胆盐能力、人工胃肠液耐受性、盐耐受性、疏水性、自聚性和抑菌能力测定，筛选得到一株体外降解胆固醇能力为42.7%的植物乳杆菌（Lactiplantibacillus plantarum）LAB4。该菌具有一定的耐酸、耐胆盐能力，在含2% NaCl的培养基中可正常生长，在经模拟人工胃、肠液处理后其活菌数均能达到108 CFU/mL。LAB4具有较好的肠道定殖潜力，其发酵上清液对4种常见食源性致病菌均表现出中度抑菌活性。综上，植物乳杆菌LAB4益生性能优良，可作为开发降胆固醇功能性食品的潜力菌株。
Isolation and characterization of cholesterol-lowering lactic acid bacteria from pickle juice
GAO Yu, LIAO Caiyu, TANG Ke, CHENG Daomei, ZHAO Changsong, LIU Xin, ZHANG Chi, HAN Yunlei
1. School of Public Health, Chengdu Medical College, Chengdu 610500, China;
2. Preclinical Medicine School, Chengdu Medical College, Chengdu 610500, China
Abstract: In order to screen lactic acid bacteria with cholesterol-lowering ability and excellent probiotic properties, 103 strains of lactic acid bacteria were isolated from 28 Sichuan traditional pickle juice. From the in vitro cholesterol degradation ability, acid resistance and bile salt resistance, artificial gastrointestinal fluid tolerance, salt tolerance, hydrophobicity, self-aggregation, and antibacterial ability Lactiplantibacillus plantarum LAB4 with an in vitro cholesterol degradation ability of 42.7% was isolated. Moreover, Lactiplantibacillus plantarum LAB4 showed promising probiotic characteristics with remarkable tolerance to pH, bile salt and 2% NaCl. Furthermore, the strain could reach 108 CFU/mL after incubation in artificial gastrointestinal fluid and possesses good potential for adhesion and colonization of intestinal epithelial cells; LAB4 showed good intestinal colonization potential, and its fermentation supernatant showed moderate antibacterial activity against four common food-borne pathogens. In summary, Lactiplantibacillus plantarum LAB4 can be used as a potential strain for developing cholesterol-lowering functional foods.
Keywords: cholesterol lowering;lactic acid bacteria;pickles;probiotics;screening
2023, 49(11):132-140 收稿日期: 2023-01-03;收到修改稿日期: 2023-02-24
基金项目: 四川省卫生健康委普及应用项目（17PJ439）；成都医学院研究生创新基金项目（YCX2022 -01-38）
作者简介: 高宇(1993-),女,湖北十堰市人,硕士研究生,专业方向为营养与健康管理。
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