作者：赵旭, 石莲花, 武利庆, 金有训
作者单位：中国计量科学研究院, 北京 100029
关键词：禽白血病;p27蛋白;同位素稀释质谱法;标准物质
摘要：
为了临床检验中p27蛋白含量测定结果的量值溯源，制备禽白血病p27蛋白冻干粉标准物质。建立同位素稀释质谱法对纯品禽白血病p27蛋白含量进行测定，结果表明，该方法准确、可靠，可以作为p27蛋白标准物质的定值方法。同时结合多家实验室定值，最终定值结果为（1.46 ± 0.13） mg/mL。该标准物质均匀性良好，在–20 ℃、4 ℃和室温环境下可以稳定7天，在–70 ℃环境中可以保存6个月。禽白血病p27蛋白冻干粉标准物质的研制对禽白血病检测的质量控制、不同检测方法间的评价具有很好的实用价值。
Development of avian leukosis virus p27 protein lyopgilized powder reference material
ZHAO Xu, SHI Lianhua, WU Liqing, JIN Youxun
National Institute of Metrology, China, Beijing 100029, China
Abstract: In order to meet the requirements of traceability of clinical p27 protein measurement results, reference materials of avian leukosis virus p27 protein lyophilized powder were developed. The value of the reference materials of avian leukosis virus p27 protein lyophilized powder were evaluated by HPLC-IDMS/MS in several laboratories, and the certified values were (1.46 ± 0.13) mg/mL. The developed method is accurate and reliable, it is able to be applied for the development of p27 protein reference material. The result indicated that the reference materials were sufficient homogeneity and had good stability, could be stored for up to 7 days at –20 ℃, 4 ℃ and room temperature. It could be stored for 6 months at –70 ℃. The development of p27 protein reference material is important for quality control of avian leukemia assays and for the evaluation of different avian leukemia assays.
Keywords: avian leukosis;p27 protein;isotope dilution mass spectrometry;reference material
2023, 49(11):166-175 收稿日期: 2023-03-07;收到修改稿日期: 2023-04-16
基金项目: 国家重点研发计划项目（2022YFC2009600，2022YFC2009602）
作者简介: 赵旭(1997-),男,新疆额敏县人,硕士研究生,专业方向为蛋白质测量技术与计量。
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