作者：侯鹏, 董玉晶, 刘磊, 夏春辉, 李爽

作者单位：齐齐哈尔医学院药学院, 黑龙江 齐齐哈尔 161006

关键词：硫化氢;香豆素;荧光检测;探针

摘要：

该文基于光诱导电子转移（PET）机制，利用H2S诱导探针分子结构中的2，4-二硝基苯醚水解，抑制PET过程，合成一种反应型H2S荧光探针。利用NMR和MS对探针1的结构进行表征。通过光谱学测试和生物学细胞实验证实，相对于其他测试物质（阴、阳离子及生物分子），它对H2S具有良好的选择性和灵敏度，可以在生理条件下检测H2S。当H2S加入到探针溶液后，溶液呈现出绿色荧光，在490 nm荧光强度恢复75倍，当探针的浓度为10.0 mol/L时，其对H2S的检测限为3.010-8 mol/L；同时，探针成功实现细胞内H2S的荧光成像，为其在生物学及医学中的实际应用奠定实验基础。

Synthesis and application of reactive hydrogen sulfide fluorescent probe based on coumarin derivarives

HOU Peng, DONG Yujing, LIU Lei, XIA Chunhui, LI Shuang

College of Pharmacy, Qiqihar Medical University, Qiqihar 161006, China

Abstract: In this paper, based on the photoinduced electron transfer (PET) mechanism, a reactive H₂S fluorescent probe was successfully synthesized by H₂S induced the hydrolysis of 2, 4-dinitrophenyl ether to inhibit PET process. The structure of probe 1 was characterized by NMR and MS. Compared with other test material (anion, ion and biological molecules), it has good selectivity and sensitivity for H₂S probe to detect H₂S under physiological conditions. With the addition of H₂S, the solution showed green fluorescence and the fluorescence intensity restore 75-fold at 490 nm. When the concentration of the probe is 10.0 μmol/L, the detection limit of H₂S is 3.0×10^-8 mol/L. At the same time, the probe of H₂S in cell fluorescence imaging showed that it would play a experimental foundation for the practical application in biology and medicine.

Keywords: hydrogen sulfide;coumarin;fluorescence detection;probe

2018, 44(2): 56-61  收稿日期: 2017-10-23;收到修改稿日期: 2017-11-26

基金项目: 2016年度黑龙江省教育厅基本科研业务费项目（科技类）（2016-KYYWF-0855）

作者简介: 侯鹏(1982-),女,黑龙江齐齐哈尔市人,讲师,博士,研究方向为有机小分子荧光探针的合成与应用。

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