作者：詹雁, 阮佳, 谭镭, 徐超群

作者单位：四川省中医药科学院, 四川 成都 610041

关键词：决明子; 橙黄决明素; 大黄素; 大黄酚; 大黄素甲醚; 超高效液相色谱法

摘要：

建立UPLC同时测定决明子中橙黄决明素、大黄素、大黄酚和大黄素甲醚含量的方法,采用ACQUITY UPLC BEH-C18色谱柱(2.1 mm50 mm1.7 m),乙腈和0.1%磷酸水溶液为流动相,采用梯度洗脱,流速为0.6 mL/min,检测波长为284 nm。结果表明橙黄决明素、大黄素、大黄酚和大黄素甲醚分别在3.002~30.02 g/mL、2.500~25.00 g/mL、10.430~104.30 g/mL和5.916~59.16 g/mL的浓度范围内均有良好的线性关系(r=0.999 9),回收率均大于95%,RSD均小于2%。该方法快速,准确,重现性好,可用于测定决明子中橙黄决明素、大黄素、大黄酚和大黄素甲醚的含量。

Simultaneous determination of aurantio-obtusifolin, emodin, chrysophanol and physcione in Semen Cassiae with UPLC

ZHAN Yan, RUAN Jia, TAN Lei, XU Chao-qun

Sichuan Academy of Chinese Medicine Sciences, Sichuan Chengdu 610041, China

Abstract: A method was established for simultaneously determining aurantio-obtusifolin, emodin, chrysophanol and physcione in Semen Cassiae by UPLC. The optimal conditions of separation and detection were achieved on an ACQUITY UPLC BEH-C18 column (2.1 mm×50 mm×1.7 μm). Mobile phase was acetonitrile and 0.1% phosphoric acid with gradient elution. Flow rate was 0.6 mL/min and detection wavelength was 284 nm. The results showed that there were good linear relationships (r=0.999 9) when the concentration of aurantio-obtusifolin, emodin, chrysophanol and physcione were in the range of (3.002-30.02)μg/mL, (2.500-25.00)μg/mL, (10.430-104.30)μg/mL, and(5.916-59.16)μg/mL respectively, the recovery rates were more than 95%, and the RSD were less than 2%. This method was simple, rapid, accurate, and reproducible, and it can be used for determining the concentration of aurantio-obtusifolin, emodin, chrysophanol and physcione in Semen Cassiae.

Keywords: Semen Cassiae; aurantio-obtusifolin; emodin; chrysophanol; physcione; UPLC

2013, 39(2): 56-58  收稿日期: 2012-8-15;收到修改稿日期: 2012-10-9

基金项目: 四川省科研院所成果转化资金项目(11010120)

作者简介: 詹雁(1980-),女,四川成都市人,助理研究员,硕士,主要从事药物分析科研工作。

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