作者：钱帆, 付玉丽, 程秀梅, 廖秀林, 杨秀培

作者单位：西华师范大学化学化工学院, 四川 南充　637000

关键词：阿霉素; 十六烷基三甲基溴化铵; 荧光光谱法; 兔血

摘要：

基于阳离子表面活性剂十六烷基三甲基溴化铵(CTAB)对蒽环类抗生素阿霉素的荧光强度有增敏作用,通过考察CTAB浓度、缓冲液pH及浓度、有机溶剂等对阿霉素荧光强度的影响,建立胶束增敏荧光光度法测定阿霉素的新方法。在体系为17 mmol/L的硼酸缓冲液(pH 6.0)且含60%(v/v)乙醇、1.9 mmol/L CTAB的条件下,阿霉素在0.5~120 ng/mL浓度范围内荧光强度呈良好的线性(r=0.999 2),检出限(3)为0.161 ng/mL。该方法用于测定兔血样中阿霉素时,回收率在93.7%~105.6%之间,相对标准偏差小于1.5%。结果表明该方法具有较好的准确度与灵敏度,可用于临床监测。

Micelle-enhanced spectrofluorimetric determination of doxorubicin in rabbit serum

QIAN Fan, FU Yu-li, CHENG Xiu-mei, LIAO Xiu-lin, YANG Xiu-pei

College of Chemistry and Chemical Engineering, China West Normal University, Nanchong 637000, China

Abstract: Doxorubicin is a kind of anthracycline antibiotics and the fluorescence of it can get enhanced in the presence of hexadecyl trimethyl ammonium bromide (CTAB) in ethanol-borate buffer solution. A new fluorescent spectrophotometry based on this principle has been developed to determine doxorubicin in rabbit serum. Method development included study of the effects of buffer pH, buffer concentration, organic solvent and CTAB concentration on the fluorescence intensity of DOX. The optimum conditions were pH 6.0 17 mmol/L borate buffer, 60% v/v ethanol and 1.9 mmol/L CTAB. The fluorescence intensity was linearly related to the concentration of doxorubicin in the range of 0.5 to 120 ng/mL with a correlation coefficient of 0.999 2 under the optimum conditions. The detection limit(3σ) is 0.161 ng/mL. The proposed method was satisfactorily applied to the determination of doxorubicin in rabbit serum. The recovery was within the range of 93.7%-105.6% (RSD<1.5%). The results showed that this method had grcat accuracy and sensitivity, which is widely applied in chinical test.

Keywords: doxorubicin; CTAB; fluorescent spectrophotometry; rabbit serum

2013, 39(2): 44-47  收稿日期: 2012-9-11;收到修改稿日期: 2012-10-13

基金项目: 国家自然科学基金项目(21277109)四川省高校科技创新团队建设项目(2010008)

作者简介: 钱帆(1990-),男,湖北襄樊市人,硕士研究生,专业方向为毛细管电泳及生物分析技术。

参考文献

[1] De B E L, Bottone A E, Voest E E. Doxorubicin and mechanical performance of cardiac trabeculae after acute and chronic treatment: a review[J]. Eur J Pharmacol,2001,415(1):1-11.
[2] Herman A H, Rahman H, Ferrans V J, et al. Prevention of chronic doxorubicin cardiotoxicity in beagles by liposomal encapsulation[J]. Cancer Res,1983,43(11):5427-5432.
[3] Trevisan M G, Poppi R J. Determination of doxorubicin in human plasma by excitation-emission matrix fluorescence andmulti-way analysis[J]. Anal Chim Acta,2003, 493(1): 69-82.
[4] 杨志洁,刘盛辉,叶建农,等. 浸蜡石墨电极伏安法测定抗癌药物盐酸阿霉素[J]. 分析化学,1996,24(4):471-474.
[5] 谭学才,李启隆,尚军. 阿霉素的示波极谱法[J]. 分析化学,1996,24(7):764-767.
[6] Anderson A G, Gergen J, Arriaga E A. Detection of doxorubicin and metabolites in cell extracts and in single cells by capillary electrophoresis with laser-induced fluorescence detection[J]. J Chromatogr B,2002,769(1):97-106.
[7] Álvarez-Cedrón L, Sayalero M L, Lanao J M. High-performance liquid chromatographic validated assay of doxorubicin in rat plasma and tissues[J]. J Chromatogr B,1999,721(2):271-278.
[8] 许金钩,王尊本. 荧光分析法[M]. 3版. 北京:科学出版社,2006:1-2.
[9] 赵刚,胡增华,黄力明,等. 荧光光谱法测定人血浆中国产阿霉素浓度及临床药代动力学观察[J]. 云南医药,1994,15(5):377.
[10] 闫继东,辛华,郑雅娟,等. 应用荧光分光光度法测定血清及组织中阿霉素含量[J]. 中国实验诊断学,2007,11(5):596-597.
[11] 赵一兵,马学芳,郭祥群,等. 阿霉素的荧光测定研究[J].厦门大学学报:自然科学版,1993,32(6):753-757.
[12] 李凤云,陈浩然,赵凤欣,等. 荧光法测定阿霉素的血药浓度[J]. 应用化学,2011,28(3):343.
[13] 张跃华,王南平,张其平,等. β-环糊精与阿霉素相互作用荧光光谱[J]. 黑龙江医药,1996,9(5):243.
[14] 国家药典委员会. 中华人民共和国药典(二部)[M]. 北京: 中国医药科技出版社,2010:693-694.