作者：马戎, 鲍峰伟, 冯文宁, 解民, 张艳芳, 陈伟华

作者单位：河北中烟工业有限责任公司技术中心, 河北 石家庄 050035

关键词：液质联用; 游离氨基酸; 烟草; 超声

摘要：

建立液相色谱串联质谱法测定烟草中20种游离氨基酸的方法。烟草样品经0.1%的盐酸溶液超声萃取并离心后,直接进样测定。色谱柱采用XTerra MS C18(50mm2.1 mm2.5 m),0.1%甲酸溶液和乙腈为流动相。结果表明:20种氨基酸的检出限为0.001~0.011 g/mL,标准曲线的拟合度均大于0.999,回收率在86.4%~105.9%之间。该方法操作简单,灵敏度高,适用于烟草中游离氨基酸的检测。

Determination of free amino acids in tobacco by liquid chromatography tandem mass spectrometry

MA Rong, BAO Feng-wei, FENG Wen-ning, XIE Min, ZHANG Yan-fang, CHEN Wei-hua

China Tobacco Hebei Industrial Corporation Technology Center, Shijiazhuang 050035, China

Abstract: A method for determining of 20 free amino acids in tobacco was developed with liquid chromatography tandem mass spectrometry. Tobacco samples were extracted with 0.1% hydrochloric acid solution by ultrasonic. After centrifugal separation treatment, they were injected into the instrument and determined divectly. The LC separation was carried on a reversed-phase XTerra MS C18(50 mm×2.1 mm×2.5 μm), 0.1% formic acid solution and acetonitrile were taken as mobile phase. The results showed that the limits of detection of 20 amino acids were in the range of 0.001-0.011 μg/mL, the linear correlation coefficients were above 0.999 in all cases, and the the recovery were in the range of 86.4%-105.9%. This method is simple operation, high sensitivity, and suitable for determining the free amino acid in tobacco.

Keywords: LC/MS; free amino acid; tobacco; ultrasonic

2013, 39(2): 34-37  收稿日期: 2012-10-19;收到修改稿日期: 2012-12-5

基金项目: 

作者简介: 马戎(1979-),男,河北唐山市人,工程师,硕士,主要从事烟草研发与烟草化学研究工作。

参考文献

[1] 闫克玉. 烟草化学[M]. 郑州:郑州大学出版社,2002:57-67.
[2] 冯雷,陈章玉,王保兴,等. 柱前衍生化反相高效液相色谱法测定烟草中的游离氨基酸[J]. 云南大学学报:自然科学版,2003(S1):241-245.
[3] 景延秋,高玉珍,鲁平,等. 反相高效液相色谱法在测定白肋烟烟叶游离氨基酸含量中的应用[J]. 河南农业大学学报,2007(5):502-505.
[4] 李丹,黄龙,朱巍,等. 反相高效液相色谱法测定烟叶中的游离氨基酸[J]. 烟草科技,2003(2):20-24.
[5] 程勇,陈玲,邓晓春. 柱前衍生HPLC法测定烟叶中20种游离氨基酸含量[J]. 烟草科技,2010(8):34-40.
[6] YC/T 282-2009烟叶.游离氨基酸的测定.氨基酸分析仪法[S].
[7] 张峻松,贾春晓,李炎强,等. 烟草中游离氨基酸的自动分析仪测定[J]. 烟草科技,2004(8):26-32.