作者：李兰英, 许丽, 徐勤, 闻艳丽, 丁敏, 刘刚

作者单位：上海市计量测试技术研究院, 上海 201203

关键词：标准物质; 盐酸克伦特罗; 猪尿冻干粉; 食品安全

摘要：

研制一套4种浓度水平的猪尿冻干粉中盐酸克伦特罗标准物质,以健康安全的猪尿作为空白基体,利用添加的方法,经过均匀性和稳定性检验,由8家实验室采用液相色谱-串联质谱法对该套标准物质进行协同定值,并进行不确定度评估。结果表明该套标准物质均匀性良好,在4℃条件下保存稳定性在1年以上,标准物质中CLB的含量及不确定度分别为:0.650.15,1.280.44,3.780.46,11.51.8 ng/mL(k=2)。该标准物质可应用于猪尿中盐酸克伦特罗检测过程的方法验证和检测结果质量控制等。

Development of reference materials of clenbuterol hydrochloride in swine urine lyophilisate

LI Lan-ying, XU Li, XU Qin, WEN Yan-li, DING Min, LIU Gang

Shanghai Institute of Measurement and Testing Technology, Shanghai 201203, China

Abstract: Reference materials (RMs) of clenbuterol hydrochloride (CLB) in lyophilized swine urine powder were developed by spiking CLB stock solution into healthy, safe and CLB-free swine urine. The homogeneity and the stability of the RMs were strictly inspected. The concentration of the RMs was certified using LC-MS/MS method by 8 different laboratories, and the uncertainty evaluation was performed finally. The analysis results showed that the RMs were homogeneous and stable for at least one year in 4℃. The certified value and expanded uncertainty of the RMs were as following: 0.65±0.15, 1.28±0.44, 3.78±0.46, 11.5±1.8 ng/mL respectively (k=2). These RMs were intended for CLB analysis method validation and quality control.

Keywords: reference material; clenbuterol hydrochloric; swine urine powder; food safety

2014, 40(2): 49-52  收稿日期: 2013-4-18;收到修改稿日期: 2013-6-13

基金项目: 国家自然科学基金青年基金项目(21205079);科技部质检行业公益项目(201310016);国家质检总局科研计划项目(2012QK273);上海市质监局科研项目(2012-04)

作者简介: 李兰英(1984-),女,山东阳谷县人,工程师,硕士,主要从事兽药残留基体标准物质的研制和生物传感器的研究。

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