作者：李涵¹, 高运华², 吴迪¹, 李倩琪¹, 杨屹¹

作者单位：1. 北京化工大学, 北京 100029;
2. 中国计量科学研究院, 北京 100013

关键词：鸡精; 基体; 呈味核苷酸; 含量; 标准物质; 不确定度评定

摘要：

以市售鸡精为基体,研制呈味核苷酸含量基体标准物质。优化鸡精中核苷酸提取条件,建立有机溶剂法提取核苷酸的技术手段。以核苷酸国家一级标准物质为标准,采用高效液相色谱外标法、离子色谱外标法两种技术对鸡精基体中呈味核苷酸特性量值进行确定,检验标准物质的均匀性和稳定性,结果表明其均匀性良好,且一年内标准物质的特性量值没有显著差异。同时建立基于离子色谱和高效液相色谱两种外标法对标准物质特性量值进行确定的不确定度评定模型,对研制的标准物质进行不确定度评定,为复杂基体中核苷酸含量标准物质的研制提供了借鉴,为呈味核苷酸检测的相关国家标准制定提供技术参考。

Study on matrix reference material of flavor nucleotides

LI Han¹, GAO Yun-hua², WU Di¹, LI Qian-qi¹, YANG Yi¹

1. Beijing University of Chemical Technology, Beijing 100029, China;
2. National Institute of Metrology, Beijing 100013, China

Abstract: The reference material of flavor nucleotides on the chicken essence matrix had been developed. The method was established for extraction the nucleotide with chloroform. The primary reference material for nucleotide had been used as the standard for certifying the value of flavor nucleotides reference materials by the high performance liquid chromatography and ionic chromatography. The homogeneity of reference materials had been tested and the result was good. The stability of reference materials has been monitored and there was no significant difference between the characteristic values within a year. A model was developed to evaluate the uncertainty of reference material of flavor nucleotides on the chicken essence matrix. This paper can be used as the reference for developing the complex matrix nucleotide content reference material and provide the technical information for national standards of nucleotide.

Keywords: chicken essence; matrix; flavor nucleotides; content; reference material; uncertainty of referenle material

2014, 40(5): 61-64  收稿日期: 2014-1-19;收到修改稿日期: 2014-3-23

基金项目: 科技部科技基础性工作专项(02-08-4GZ);国家质检总局食品安全监管专项(ASPAQ1201)

作者简介: 李涵(1987-),女,辽宁本溪市人,硕士研究生,专业方向为分析化学。

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