作者：江阳¹, 刘滔¹, 汪文家², 谢碧俊¹, 朱岚¹, 胡彬¹, 孙成均²

作者单位：1. 四川省疾病预防控制中心, 四川 成都 610041;
2. 四川大学华西公共卫生学院, 四川 成都 610041

关键词：多环芳烃化合物;加速溶剂萃取;PM2.5;高效液相色谱法

摘要：

建立大气颗粒物PM2.5中16种优控多环芳烃(PAHs)的加速溶剂萃取-高效液相色谱法同时测定方法。用中流量PM2.5采样器, 以玻璃纤维滤纸为滤料, 以100 L/min的流速连续采样24 h。采样后的玻璃纤维滤纸经正己烷-丙酮(6:4, /)于加速溶剂萃取仪中提取, 提取液浓缩后用乙腈定容, 通过C18色谱柱分离, 紫外和荧光检测器检测。16种PAHs在0.20～5.00 g/mL范围内线性相关系数r0.999 8, 检出限为0.002～0.012 g/mL, 方法平均回收率为71.3%～96.5%, 相对标准差为0.50%～4.8%。该法简便、快速、准确、灵敏, 将其应用于成都市3个区域空气颗粒物PM2.5中16种PAHs的测定, 结果理想。

Simultaneous determination of 16 polycyclic aromatic hydrocarbons in PM2.5 by accelerated solvent extraction coupled with high performance liquid chromatography

JIANG Yang¹, LIU Tao¹, WANG Wenjia², XIE Bijun¹, ZHU Lan¹, HU Bin¹, SUN Chengjun²

1. Sichuan Provincial Center for Disease Control and Prevention, Chengdu 610041, China;
2. West China School of Public Health, Sichuan University, Chengdu 610041, China

Abstract: This paper is related to the establishment of a method for simultaneously determining 16 polycyclic aromatic hydrocarbons (PAHS) in PM2.5 based on accelerated solvent extraction coupled with high performance liquid chromatography (ASE-HPLC). Atmospheric particulates were collected in a medium-volume sampler with fiberglass filter paper as filter materials at the flow of 100 L/min for 24 hours nonstop. After that, the fiberglass filter paper was extracted by adding hexane-acetone(6:4, v/v) in an accelerated solvent extraction apparatus. The extract was added with acetonitrile to form a constant volume after enrichment. The analytes were separated on a C18 column and detected by UV and fluorescence detectors. The linearly dependent coefficient of the 16 PAHs within the scope of 0.20-5.00 μg/mL was equal to and greater than 0.999 8; the detection limits ranged from 0.002 to 0.012 μg/mL; the average recoveries were from 71.3% to 96.5%; and the relative standard deviations(RSDs) were between 0.50% and 4.80%. The method has been proved to be simple and rapid, accurate and sensitive in accordance with the satisfactory results achieved during the simultaneous determination of 16 PAHS in PM2.5 in Chengdu.

Keywords: polycyclic aromatic hydrocarbons;accelerated solvent extraction;PM2.5;high performance liquid chromatography

2015, 41(6): 43-46,51  收稿日期: 2014-12-1;收到修改稿日期: 2015-2-7

基金项目: 

作者简介: 江阳(1987-),女,技师,硕士,主要从事理化检验工作。

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