作者：代晶, 石明芯, 王佳慧, 黄丹, 黄慧, 廖昌军

作者单位：成都医学院药学院, 四川 成都 610083

关键词：忍冬藤;高效液相色谱;柱温

摘要：

采用HPLC测定不同市售地、不同直径的忍冬藤药材中各活性成分的质量分数(新绿原酸、绿原酸、咖啡酸、獐牙菜苷、马钱苷、3,5-二咖啡酰喹尼酸、4,5-二咖啡酰喹尼酸)。色谱柱为Shiseido C18(4.6 mm250 mm5 m),流动相为乙腈-0.1%磷酸,并进行梯度洗脱,体积流量为1 mL/min,柱温为 35 ℃,检测波长为238,326,350 nm。结果显示,上述成分的进样质量与峰面积之间线性关系良好(r0.999 5),精密度RSD4.3%,重复性RSD4.1%,平均加样回收率在96.8%~104.1%。本方法简单、准确,适用于忍冬藤的质量控制。

Simultaneous determination of seven components in lonicerae japonicae caulis by HPLC

DAI Jing, SHI Mingxin, WANG Jiahui, HUANG Dan, HUANG Hui, LIAO Changjun

School of Pharmacy, Chengdu Medical College, Chengdu 610083, China

Abstract: An HPLC method was developed to determine simultaneously the neochlorogenic acid, chlorogenic acid, caffeic acid, sweroside, loganin, 3,5-O-dicaffeoylquinic acid and 4,5-O-dicaffeoylquinic acid in Lonicerae Japonicae Caulis. The analysis was performed on a Shiseido C18 column (4.6 mm×250 mm×5 μm), alone with acetonitril e-0.1% H3PO4 as the mobile phase and a gradient elution at the flow rate of 1 mL/min. The column temperature was maintained at 35 ℃, and the detection wavelength was set at 238, 326 nm and 350 nm. The calibration curves were linear in certain ranges for eight compounds (r>0.999 5). The precision RSD is lower than 4.3%, the repeatability RSD is smaller than 4.1%, and the average recovery ranged from 96.8% to 104.1%. This method is simple, accurate, and suitable for controlling the quality of Lonicerae Japonicae Caulis.

Keywords: lonicerae japonicae caulis;HPLC;column temperature

2015, 41(8): 44-47,55  收稿日期: 2015-4-28;收到修改稿日期: 2015-6-23

基金项目: 四川省教育厅理科重点项目(15ZA0263,12ZA026)

作者简介: 代晶(1979-),女,吉林省吉林市人,讲师,博士,主要从事药物分析与药代动力学研究。

参考文献

[1] 国家药典委员会. 中国药典(一部)[M]. 北京:中国医药科技出版社,2010:179.
[2] 崔雅璠,丁樱. 配伍应用海风藤、络石藤、忍冬藤治疗顽固性过敏性紫癜经验[J]. 辽宁中医杂志,2014,41(10):2062-2063.
[3] 刘芳,罗星,向茗,等. 刘祖贻清热解毒利湿法治疗类风湿关节炎经验[J]. 上海中医药杂志,2014,48(4):1-4.
[4] 曲卓锋. 忍冬桂芪汤对化脓性扁桃体炎的治疗体会[J].光明中医,2013,28(8):1629-1630.
[5] 刘静. 忍冬藤注射液制剂的研究[D]. 长春:吉林大学,2013.
[6] 吴德峰,王丽辉,黄玉树. 忍冬藤作为肉鸡饲料添加剂的促生长和免疫作用研究[J]. 家畜生态学报,2007,28(3):23-28.
[7] 余清,苏玲. 中药熏洗治疗糖尿病足早期病变的临床研究[J]. 中国民族民间医药,2012,21(22):113-113.
[8] Qian Z M, Li H J, Li P, et al. Simultaneous quantification of seven bioactive components in caulis lonicerae japonicae by high performance liquid chromatography[J]. Biomed Chromatogr,2007(21):649-654.
[9] 辛贵忠,钱正明,周建良,等. 忍冬藤质量标准研究[J]. 中国药学杂志,2009,44(1):52-54.
[10] 纪瑞锋,刘素香,刘毅,等. HPLC法测定不同产地忍冬藤中4种环烯醚萜苷[J]. 药物评价研究,2013,36(4):289-292.
[11] 曹君,尼锟潭,叶诗佳. 快速微乳电动毛细管色谱法同时测定忍冬藤中11种有效成分[J]. 2010,38(5):765-765.
[12] 苟占平,万德光. 分光光度法测定6种忍冬藤中绿原酸的含量[J]. 时珍国医国药,2007,18(8):1900-1901.
[13] 王书妍,翁慧,张力,等. 忍冬藤挥发油化学成分GC/MS 分析[J]. 内蒙古民族大学学报:自然科学版,2011,26(1):18-20.