作者：史健阳1, 宁华2, 徐冉3, 高珍璇3, 余洋3, 刘保3, 柯永培4, 孙群1,3
作者单位：1. 四川大学轻纺与食品学院, 四川 成都 610064;
2. 四川省自然资源科学研究院, 四川 成都 610065;
3. 四川大学生命科学学院, 四川 成都 610064;
4. 四川农业大学农学院, 四川 成都 611130
关键词：气体ClO2;黄曲霉菌;黄曲霉毒素B1;玉米
摘要：
该文系统研究平板条件下二氧化氯（ClO2）对黄曲霉菌丝生长、孢子萌发，以及黄曲霉菌产毒能力的抑制作用，考察ClO2对高水分玉米中黄曲霉菌孢子萌发的影响。结果表明：0.60 mg/L ClO2作用40 min对黄曲霉菌丝生长抑制率为9.78%，但对黄曲霉毒素B1（AFB1）生成的抑制率达78.22%；0.75 mg/L ClO2作用20 min即可完全抑制黄曲霉菌丝生长和AFB1产生，因此为最低有效浓度；而1.00 mg/L和1.50 mg/L ClO2作用5 min对黄曲霉菌孢子萌发抑制率分别达到53.38%和100%；以aw0.92的玉米为载体，9.0 mg/L的ClO2作用60 min对黄曲霉菌孢子萌发抑菌率可达86.63%。因此，ClO2对黄曲霉菌丝生长、产毒能力、孢子萌发都有强烈抑制作用，对抑制高水分玉米中黄曲霉菌孢子萌发也有显著效果，有潜力防控仓储前玉米中黄曲霉菌生长和AFB1污染。
Inhibitory effect on the growth and aflatoxin-production of Aspergillus flavus by ClO2 and its application in corn of high water content
SHI Jianyang1, NING Hua2, XU Ran3, GAO Zhenxuan3, YU Yang3, LIU Bao3, KE Yongpei4, SUN Qun1,3
1. College of Textiles and Food Science, Sichuan University, Chengdu 610064, China;
2. Sichuan Provincial Institute of Nature Resource Science, Chengdu 610065, China;
3. College of Life Science, Sichuan University, Chengdu 610064, China;
4. Agronomy College, Sichuan Agricultural University, Chengdu 611130, China
Abstract: The efficacy of gaseous chlorine dioxide (ClO2) on the inhibition of mycelial growth and spore germination of Aspergillus flavus,as well as aflatoxin-producing ability were evaluated.The effect of ClO2 on the germination of A.flavus in maize of aw 0.92 was also evaluated.Results demonstrated that ClO2 noticeably suppressed A.flavus growth,sporulation,and AFB1 producing.After the treatment of 0.75 mg/L ClO2 for 20 min,the growth of A.flavus was completely inhibited,while the inhibition rates of ClO2 at 0.60 mg/L for 40 min was 9.78% and 78.22% for mycelium formation and AFB1 development,respectively.On spore germination,after treatment of 1.00 and 1.50 mg/L ClO2 for 5 min,the inhibition rates reached 53.38% and 100%,respectively.In addition,the treatment of 9.0 mg/L ClO2 for 60 min on corn (aw 0.92) resulted in the inhibition rate of 86.63% for spore germination.Accordingly,chlorine dioxide has the potential to be applied for controlling the growth of A.flavus and the pollution of AFB1 in corn.
Keywords: gaseous ClO2;Aspergillus flavus;aflatoxin B1;corn
2017, 43(10): 47-52 收稿日期: 2017-06-10;收到修改稿日期: 2017-07-25
基金项目: 四川省科技支撑计划项目（2016SZ0068）
作者简介: 史健阳(1992-),女,甘肃平凉市人,硕士研究生,专业方向为粮食、油脂及植物蛋白工程。
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