作者：赵爱平^1,2, 冯德建^1,2, 许洋^1,2, 邹燕^1,2, 张琦³, 李怀平^1,2

作者单位：1. 中国测试技术研究院, 四川 成都 610021;
2. 茶叶标准与检测技术四川省重点实验室, 四川 成都 610021;
3. 四川省食品药品检验检测院, 四川 成都 611731

关键词：蚕豆保健品;左旋多巴;高效液相色谱仪;蚕豆粉;蚕豆茶

摘要：

建立蚕豆保健品中左旋多巴含量的高效液相色谱测定方法。样品经0.10 mol/L盐酸溶液超声提取20 min，XDB C18色谱柱分离，以甲醇-10 mmol/L磷酸二氢钾溶液（体积比2：98）等度洗脱，280 nm下检测，外标法定量。结果表明：左旋多巴质量浓度在1.656~265.0 mg/L范围内与峰面积呈现良好的线性关系，方法的平均加标回收率为98.4%~100.9%，相对标准偏差为0.83%~2.18%，检出限为1.9 mg/kg，定量限为6.3 mg/kg。能够满足蚕豆保健品中左旋多巴含量的检测和质量控制需要。

Study on the determination of L-Dopa in faba bean dietary supplement

ZHAO Aiping^1,2, FENG Dejian^1,2, XU Yang^1,2, ZOU Yan^1,2, ZHANG Qi³, LI Huaiping^1,2

1. National Institute of Measurement and Testing Technology, Chengdu 610021, China;
2. Standard and Testing Technology of Tea Key Laboratory of Sichuan Province, Chengdu 610021, China;
3. Sichuan Institute for Food and Drug Control, Chengdu 611731, China

Abstract: A method was developed for the determination of L-Dopa in faba bean dietary supplement by HPLC. After extracted by Ultrasonic-assisted with 0.10 mol/L hydrochloric acid solution for 20 minutes, the sample was separated on a XDB C18 column with the isocratic elution of methanol and 10 mmol/L potassium phosphate monobasic solution(2:98, V/V) as mobile phase. The detection wavelength was 280 nm and the sample was quantified by the external standard method. The results showed that a good linearity was obtained for L-Dopa concentration in the range of 1.656-265.0 mg/L, the average recoveries of L-Dopa ranged from 98.4% to 100.9% and the RSDs were 0.83%-2.18%. The detection limit and quantitation limit of L-Dopa were 1.9 mg/kg and 6.3 mg/kg, respectively. The method is suitable for the determination and quality control of L-Dopa in faba bean dietary supplement.

Keywords: faba bean dietary supplement;L-Dopa;HPLC;faba bean powder;faba bean tea

2018, 44(3): 57-60  收稿日期: 2017-10-17;收到修改稿日期: 2017-11-23

基金项目: 国家重点研发计划项目（2016YFF0202300）

作者简介: 赵爱平(1975-),女,河南内黄县人,工程师,主要从事食品质量检测技术与标准研究。

参考文献

[1] 郑卓杰. 中国食用豆类学[M]. 北京:中国农业出版社,1997:53-91.
[2] 杨生华,刘荣,杨涛,等. 蚕豆种质资源种子表型性状精准评价[J]. 中国蔬菜,2016(10):32-40.
[3] 郑开斌,李爱萍,廖素凤,等. 蚕豆不同部位左旋多巴(L-Dopa)含量研究[J]. 福建农业学报,2012,27(4):333-336.
[4] 耿贵工. 大田蚕豆各器官中左旋多巴含量的分析[J]. 北方园艺,2012(2):26-27.
[5] 耿贵工,刘玉皎,杨希娟. 蚕豆生长期L-DOPA含量的分析[J]. 食品工程,2012(3):120-122.
[6] 巫世红,蒋伟哲,吕立,等. 猫豆药材的质量标准研究[J]. 中药材,2009,32(3):333-335.
[7] CHEN X F, ZHANG J Y, ZHAI H L, et al. Determination of levodopa by capillary zone electrophoresis using an acidic phosphate buffer and its application in the analysis of beans[J]. Food Chemistry,2005,92(2):381-386.
[8] 郑开斌,李爱萍,曹奕鸯,等. 蚕豆花左旋多巴液相色谱检测方法的建立[J]. 江苏农业学报,2012,28(3):688-690.
[9] 赵永成. HPLC法测定狗爪豆中的左旋多巴[J]. 中草药,2001,,32(6):512-513.
[10] 黄海滨,苏健,谭叶憧. HPLC法测定藜豆中左旋多巴的含量[J]. 广西植物,2004,24(5):460-462.
[11] KASTURE V S, SONAR V P, PATIL P P, et al. Quantitative estimation of L-Dopa from polyhebal formulation by using RP-HPLC[J]. American Journal of Pharmtech Research,2014,4(3):408-414.
[12] RATHOD B G, PATEL N M. Development of validated RP-HPLC method for the estimation of L-Dopa from Mucuna pruriens, its extracts and in Aphrodisiac formulation[J]. International Journal of Pharma Sciences and Research,2014,5(8):508-513.
[13] 邓霖芳,谭蓓蓓,窦冕,等. HPLC同时测定猫豆中左旋多巴及其衍生物含量[J]. 中国现代应用药学,2016,33(7):845-848.