作者：李姝, 李宁浙, 陈冲, 齐盼盼, 宋丽菊, 吴道艳, 刘明栋, 汪学阁, 赵建

作者单位：四川大学生命科学学院资源微生物及生物技术重点实验室, 四川 成都 610064

关键词：有柄石韦;内生真菌;抑菌活性;代谢物;GC-MS

摘要：

以药用植物有柄石韦叶为材料,采用组织块法与微量稀释法分离并筛选出一株具有抑菌活性的内生真菌S-34。经形态学观察、ITS测序与序列比对鉴定内生真菌S-34为链格孢属(Alternaria sp.)。进一步利用GC-MS对其代谢物进行检测与分析,结果显示:内生真菌S-34发酵液乙酸乙酯提取物的抑菌成分主要为氟苯甲酸(20.86%)、苯乙醇(4.89%)、2,3-丁二醇(3.64%),菌体乙酸乙酯提取物的抑菌成分主要为苯甲醛(2.27%)、亚麻醇(2.53%)、亚油酸单甘油酯(1.69%)、亚麻酰氯(9.28%)、反式角鲨烯(7.26%)、麦角甾醇(2.78%)。研究表明,有柄石韦内生真菌S-34的代谢物中蕴藏着较为丰富的抑菌活性物质,是开发天然药物的潜在资源。

Identification of an endophytic fungus S-34 isolated from Pyrrosia petiolosa and analysis of its metabolites

LI Shu, LI Ningzhe, CHEN Chong, QI Panpan, SONG Liju, WU Daoyan, LIU Mingdong, WANG Xuege, ZHAO Jian

Key Laboratory of Microbiological Resource and Technology, College of Life Sciences, Sichuan University, Chengdu 610064, China

Abstract: An endophytic fungus S-34 which has antimicrobial activity was isolated from the leaves of the Chinese medicinal plant P. petiolosa through a tissue separating method and a microdilution method. The endophytic fungus S-34 was identified as Alternaria sp. according to the morphologic observation and ITS sequence analysis. Its metabolites were further studied with GC-MS. The findings show that the main antibacterial components of the fermentation liquid of S-34 extracted with ethyl acetate were fluorobenzoic acid (20.86%), phenylethyl alcohol (4.89%), 2, 3-butanediol(3.64%). Besides, the main antibacterial components of the mycelia ethyl acetate extract were benzaldehyde (2.27%), linoleny alcohol (2.53%), 9, 12-octadecadienoicacid (Z, Z)-2, 3-dihydroxypropyl ester(1.69%), 9, 12-octadecadienoylchloride,(Z, Z)-(9.28%), 2, 6, 10, 14, 18, 22-tetracosahexaene, 2, 6, 10, 15, 19, 23-hexamethyl-,(all-E)-(7.26%), neoergosterol (2.78%). As indicated in the study above, the endophytic fungus S-34 of P. petiolosa contains plenty of antibacterial substances as potential resources for the development of natural medicines.

Keywords: P. petiolosa;endophytic fungi;antimicrobial activities;metabolite;GC-MS

2016, 42(5): 61-65,97  收稿日期: 2016-01-15;收到修改稿日期: 2016-02-13

基金项目: 国家自然科学基金(31270175);教育部新世纪人才和川大优秀青年学者项目(NCET-13-0397,2013SCU04B14)

作者简介: 李姝(1991-),女,湖北宜昌市人,硕士研究生,专业方向为资源微生物应用。

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