作者：谭和平1,2, 周黎黎1,2, 徐文平1,2, 赵爱平1,2, 谭福元1,2
作者单位：1. 中国测试技术研究院, 四川 成都 610021;
2. 茶叶标准与检测四川省重点实验室, 四川 成都 610021
关键词：梯度; 离子色谱法; 茶叶; 无机阴离子
摘要：
建立了一种利用强疏水性分离柱,通过KOH梯度淋洗,同时测定茶叶中F-、Cl-、NO3-、PO43-、SO42-、BrO3-、Br-、NO2-的方法。采用沸水冲泡茶样,茶水比为1:50,时间为5 min,过滤后稳定30 min待测;样液经过活性炭净化,再用0.20 m滤膜过滤后待测。结果表明:8种无机阴离子色谱峰能达到完全的基线分离,且浓度与峰面积呈良好的线性关系,检出限均低于0.056 mg/L,加标回收率为96.5%~105.1%,该法用于茶样中8种无机阴离子含量的测定,结果稳定且重复性较好。
Simultaneous determination of F-, Cl-, NO3-, PO43-, SO42-, BrO3-, Br- and NO2- in tea by IC with hydrophobic column AS-15
TAN He-ping1,2, ZHOU Li-li1,2, XU Wen-ping1,2, ZHAO Ai-ping1,2, TAN Fu-yuan1,2
1. National Institute of Measurement and Testing Technology, Chengdu 610021, China;
2. Standard and Testing Technology of Tea Key Laboratory of Sichuan Province, Chengdu 610021, China
Abstract: Based on gradient elution method, ion chromatography(IC) with the hydrophobic column AS-15 was developed to simultaneously determine the content of inorganic anions (i.e., F-, Cl-, NO3-, PO43-, SO42-, BrO3-, Br- and NO2-) in tea. Firstly, the tea powders were soaking in boiling water for 5 min at the ratio of tea to water as 1:50. Then, the powders were filtered out, and the filtrate was stabilized for 30 min. The prepared solution was treated by activated carbon cartridge, and the collected solution was filtered through a 0.20 μm filter membrane before injection. The results showed that the 8 inorganic anions were thoroughly separated, the contents of anions and peak areas gave a good linear relationship, the detection limit was less than 0.056 mg/L, and their recoveries in the range from 96.5% to 105.1%. The established method is robust, and it can be used for the routine determination of inorganic anions with sound repeatability.
Keywords: gradient; ion chromatography; tea; inorganic anion
2011, 37(6): 49-52 收稿日期: 2011-3-22;收到修改稿日期: 2011-6-5
基金项目: 国家科技部科技基础专项(2004DEA71180);四川省科技支撑计划项目(2009NZ0015)
作者简介: 谭和平(1957-),男,重庆市人,研究员,享受国务院政府津贴专家,从事茶叶生物化学研究。
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