作者：杜利成

作者单位：西南科技大学分析测试中心, 四川绵阳 621010

关键词：核黄素; 电化学行为; 玻碳电极

摘要：

用循环伏安法和线性扫描伏安法探讨核黄素在玻碳电极上的电化学行为。在多种缓冲液中测试,发现HAc-NaAc作为缓冲液时电化学氧化还原峰形最好,峰电流较大,电化学过程表现出良好的可逆性,电子转移数n为2,扩散系数D0为2.310-5cm2s-1;改变缓冲液的pH值,峰电流随pH值增大而负移,说明其在电极表面有吸附作用;光照后核黄素在-0.35V和-0.45V附近出现两个还原峰;分析实验数据可得,在1.010-4mol/L数量级时还原峰峰电流与其浓度呈现良好的线性关系。

Study on electrochemical behavior of riboflavin at glassy carbon electrode

DU Li-cheng

Analysis and Testing Center, Southwest University of Science and Technology, Mianyang 621010, China

Abstract: The electrochemical behaviors of riboflavin at glassy carbon electrode were studied with cyclic voltammetry and linear sweeping voltammetry methods. It was shown that the shape of electrochemical oxidation-reduction peak and the corresponding peak current in acetic acid/sodium acetate buffer are the best and strongest among a variety of buffers. The reversibility of the electrochemical behavior for riboflavin at glassy carbon electrode was found to be good, and the electron transfer number, n, was calculated to be 2 and the diffusion coefficient D₀ is 2.3×10^-5 cm²·s^-1. The average peak current of riboflavin was rectilinear to the pH value of acetic acid/sodium acetate buffer, which indicates that riboflavin has certain adsorbability at the surface of the glassy carbon electrode. When the riboflavin has been exposed to light, two reduction potentials,-0.35 V and -0.45 V have been found. The experiment data indicate that the peak current is rectilinear to riboflavin concentration in the order of 1.0×10^-4 mol/L.

Keywords: Riboflavin; Electrochemical behavior; Glassy carbon electrode

2008, 34(6): 81-85  收稿日期: 2008-1-4;收到修改稿日期: 2008-3-26

基金项目: 

作者简介: 杜利成(1964-),男,四川绵阳市人,副教授,硕士,长期从事无机化学、分析化学及有机化学的教学与研究。

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