作者：谭和平, 叶善蓉, 陈丽, 邹燕

作者单位：中国测试技术研究院, 四川成都 610021

关键词：茶叶; 有机酸; 分析; 高效液相色谱法; 离子色谱法

摘要：

针对茶叶中有机酸测定前处理分析测试条件等不统一,导致测试结果无可比性的问题,该文在参阅1907年以来国内外关于茶叶中有机酸分析的30余篇文献的基础上,对茶叶中有机酸的分析方法做了较系统的归纳阐述,并对目前常用的分析方法包括高效液相色谱法、离子色谱法以及毛细管电泳色谱法等进行了评述,提出了茶叶中有机酸测定应进行标准化,以确保测试结果的可比性。

Determination Overview of organic acids in tea

TAN He-ping, YE Shan-rong, CHEN Li, ZOU Yan

National Institute of Measurement and Testing Technology, Chengdu 610021, China

Abstract: The measurement results of organic acids in tea were incomparable for the different pre-treatments and test conditions. More than 30 literatures since 1907 about the organic acid determinations in tea had been consulted. And these determination methods of organic acids in tea were introduced in detail. The most general methods at present such as high performance liquid chromatography, ion chromatography, capillary electrophoresis, and so on were emphasized. Based on which, the determination method of organic acids in tea should be standardized to improve the comparability of the measurement results.

Keywords: Tea; Organic acids; Analysis; HPLC; IC

2008, 34(6): 77-80  收稿日期: 2008-5-18;收到修改稿日期: 2008-7-25

基金项目: 国家科技基础条件平台(2004DEA71180)

作者简介: 谭和平(1957-),男,重庆市人,研究员,主要从事茶树生理生化研究。

参考文献

[1] 陈文峰,屠幼英,吴媛媛,等.黑茶紧压茶浸提物对胰蛋白酶活性的影响[J].中国茶叶,2002,24(3):16-17.
[2] 杜继煜,白岚,白宝璋.茶叶的主要化学成分[J].农业与技术,2003,23(1):53-55.
[3] 尹方.茶叶中的有机酸叙要及其草酸根测定[J].茶业通报,1980(4):27-28.
[4] 国家药典委员会.中华人民共和国药典[M].北京:化学工业出版社,2000.
[5] Wipawee P,Eiichiro F,Takeshi B,et al. Prediction of Japanese green tea ranking by gas chromatography/mass spectrometry-based hydrophilic metabolite fingerprinting[J]. Journal of Agricultural and Food Chemistry, 2007,55(2):231-236.
[6] 张光军,许建文,丁明玉.离子交换色谱法同时分析绿茶中有机酸和无机阴离子的含量[J]. 新乡医学院学报, 2002,19(1):36-38.
[7] Innocenzo G,Casella,GattaM.Determination of electroactive organic acids by anion-exchange chromatography using a copper modified electrode[J]. Journal of Chromatography A,2001(912):223-233.
[8] Ding M Y,Chen P R, Luo G A. Simultaneous determination of organic acids and inorganic anions in tea by ion chromatography[J].Journal of Chromatography A,1997,764(2):341-345.
[9] Alcazar A,Fernandez-Caceres P L,Martin M J,et al. Ion chromatographic determination of some organic acids, chloride and phosphate in coffee and tea[J]. Talanta,2003,61(2):95-101.
[10] 赵景婵,郭治安,常建华,等.有机酸类化合物的反相高效液相色谱法的分离条件研究[J]. 色谱,2001,19(3):260.
[11] 屠幼英,梁慧玲,陈暄,等.紧压茶儿茶素和有机酸的组成分析[J].茶叶,2002,28(1):22-24.
[12] 丁玲.茯砖茶加工过程中主要化学成分的变化及其对胰酶活性的影响[D].硕士学位论文.杭州:浙江大学, 2006.
[13] 丁玲,屠幼英,陈晓敏,高效液相色谱测定紧压茶中有机酸条件研究[J].茶叶,2005,31(4),224-227.
[14] Jayabalan R,Marimuthu S,Swaminathan K.Changes in content of organic acids and tea polyphenols during kombucha tea fermentation[J].Food Chemistry,2007(102):392-398.
[15] Hideki H,Yuji Y,Katsunori K. Analysis of organic anions in tea infusions using capillary electrophoresis[J]. Journal of Chromatography A,1998,817(1-2):139-144.