作者：周艳明, 李娜, 牛森, 李彩卫, 赵瑛博

作者单位：沈阳农业大学, 辽宁沈阳 110161

关键词：草甘膦; 高效液相色谱; 衍生反应; 荧光检测器; 残留

摘要：

建立了水中草甘膦残留量的高效液相色谱检测方法,方法采用柱前衍生,经阴离子交换色谱柱分离,以荧光检测器检测,并对该方法的准确性和精密度进行了试验,结果表明该方法快速、灵敏度高、精密度好。草甘膦的检出限为0.005 g/ml,各浓度水平的添加回收为85%~101%,变异系数为2.5%~4%。我国至今尚无测定草甘膦残留量的标准方法,该方法可靠、灵敏、简便可行,为制定国家标准分析方法提供依据。

Detection glyphosate residues in water by LC

ZHOU Yan-ming, LI Na, NIU Sen, LI Cai-wei, ZAO Ying-bo

Shenyang Agricultural University, Shenyang 110161, China

Abstract: A high performance liquid chromatographic method is developed for determination of glyphodate in water.The sample are derived before column,and then separated with ion-exchange,at last glyphosate has been detected by the fluorescence detection.The method was proved to be rapid,sensitive and accurate.The limit of detection was 0.005 μg/ml,Mean recoveries were from 85% to 101%,and coefficients of variation were from 2.5% to 4%.At preset standard analysis method of glyphosate has not been studiedyet.Therefore,it is essential to find a reliable,sensitive and simple method for determination of glyphosate residue.The research can provide basis for constituting standard analysis methods.

Keywords: Glyphosate; Liquid chromatographic; Derivative; Fluorescence detection; Residue

2007, 33(3): 114-116  收稿日期: 2006-11-9;收到修改稿日期: 2007-1-15

基金项目: 

作者简介: 周艳明(1955-),女,硕士,教授,主要从事农药残留分析。

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