作者：刘江生
作者单位：龙岩卷烟厂技术中心, 福建龙岩 364000
关键词：高效液相色谱; 蒸发光散射检测器; 质量控制; 烟用香精香料; 指纹图谱
摘要：
为了监控烟用香精香料的质量,应用高效液相色谱-蒸发光散射仪,Nova-Pak C18柱(4m,150mm3.9mm),以烟酸甲酯为内标,乙醇-水(71:29)为流动相,等度洗脱,流速1.0mL/min,对烟用香精香料进行HPLC分析,通过对待测样品与标准样品的指纹图谱谱峰匹配,计算指纹图谱间的相似度考察烟用香精香料质量稳定性。
Researches on quality assessment of tobacco essence perfume by HPLC-ELSED
LIU Jiang-sheng
Technology Center of Longyan Cigarette Factory, Longyan 364000, China
Abstract: In order to monitor the quality of tobacco essence perfume,the samples of tobacco essence perfume were analyzed by high performance liquid chromatography with evaporative light scattering detector (HPLC-ELSD),using unicotinsaeuremethylester as an internal standard,a Nova-Pak C18 (4μm,150mm×3.9mm) as analytical column,ethanol-acotonitrile (71:29) as the mobile phase,at a flow rate of 1.0mL/min.Matching the chromatogram peaks between the sample to be measured and the standard chromatogram fingerprint and calculating the similarity between HPLC fingerprint spectrum quality stability of the tobacco essence perfume was investigated.
Keywords: High-performance liquid chromatography; ELSD; Quality assessment; Tobacco essence perfume; Fingerprint spectrum
2007, 33(2): 128-129,140 收稿日期: 2006-5-23;收到修改稿日期: 2006-8-7
基金项目: 国家烟草专卖局科技开发项目资助(110200201017、110200201018)
作者简介: 刘江生(1969-),男,工程师,硕士,主要从事烟草化学研究。
参考文献
[1] Yang W, Ming Y.D.Analysis of Carbohydrates in Drinks by High-performance liquid Chromatography with a Dynamically Modified Amino Column and Evaporative Light Scattering Detection[J].Journal of Chromatography A, 2000, 904:113-117.
[2] 盛志艺, 吕健, 徐海涛, 等.同时蒸馏萃取-色谱分析仪在香精质量控制中的应用[C].中国烟草学会工业委员会香精香料学组年会暨学术研讨会论文集, 2002:118-121.
[3] 王玉, 徐若飞, 徐济仓, 等.烟用香精香料指纹图谱与质控体系的研究[C].中烟烟草化学学组年会暨学术研讨会论文集, 2004:248-250.
[4] 王钧, 赵曰利.色谱指纹图谱对香精香料质量控制的研究[C]. 中烟烟草化学学组年会暨学术研讨会论文集, 2004:209-212.
[5] Cai Jibo, Liu Baizhan, Ling Ping, et al.Analysis of Free and Bound Volatiles by gas Chromatography and gas Chromatography-mass Spectrometry in Uncased and Cased Tobaccos[J].J Chromat A, 2002, 947:267-275.
[6] 王龙星, 肖红斌, 梁鑫森, 等.一种评价中药色谱指纹谱相似性的新方法:向量夹角法[J].药学学报, 2002, 37(9): 713-717.
[7] 程翼宇, 陈闽军, 吴永江.化学指纹图谱的相似性测试及其评价方法[J].化学学报, 2002, 60(11):2017-2021.
