作者：谢莹1,2, 卢辛未1,2, 谭镭2, 詹雁2, 阮佳2, 徐超群2
作者单位：1. 成都中医药大学, 四川 成都 610075;
2. 四川省中医药科学院, 四川 成都 610041
关键词：马甲子;messagenic acid B;超高效液相色谱;抗肿瘤活性
摘要：
建立UPLC同时测定马甲子药材中马甲子素和messagenic acid B 2种抗肿瘤活性成分的方法。采用Waters ACQUITY UPLC BEH-C18（2.1 mm 50 mm，1.7 m）色谱柱，乙腈-0.1%甲酸溶液流动相梯度洗脱；流量：0.45 mL/min；柱温30 ℃；检测波长320 nm。结果表明：马甲子素和messagenic acid B分别在0.198 6~3.972 g，0.151 4~3.028 g范围进样量与峰面积呈现良好的线性关系，平均加标回收率分别为99.20%（RSD=1.34%）和99.69%（RSD=0.56%）。该方法能在3.5 min内使马甲子素和messagenic acid B最大程度地分离，相比较HPLC而言，更加快捷、高效。
UPLC determination of paliurusene and messagenic acid B content in Paliurus ramosissimus leaves
XIE Ying1,2, LU Xinwei1,2, TAN Lei2, ZHAN Yan2, RUAN Jia2, XU Chaoqun2
1. Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China;
2. Sichuan Academy of Chinese Medicine Sciences, Chengdu 610041, China
Abstract: A UPLC method was established for simultaneous determination of two antitumor active ingredients(paliurusene and messagenic acid B) in paliurus ramosissmus. A Waters ACQUITY UPLC BEH-C18(2.1 mm × 50 mm, 1.7 μm) column with acetonitrile -0.1% methanoic acid as a mobile phase was adopted for gradient elution at the flow rate of 0.45 mL/min, the column temperature was 30 ℃ and the detection wavelength was 320 nm． The results showed that two active ingredients (paliurusene and messagenic acid B) had a good linearity relationship between the sample content and peak area within the selected concentration range(0.198 6-3.972 μg, 0.151 4-3.028 μg)． The average sample recovery rate was 99.20%(RSD=1.34%) and 99.69%(RSD=0.56%) respectively. Two active ingredients achieved the greatest degree of separation in 3.5 min. Compared with HPLC, this method was much faster and more efficient.
Keywords: Paliurus ramosissmus;messagenic acid B;UPLC;antitumor activity
2017, 43(4): 48-51 收稿日期: 2016-08-23;收到修改稿日期: 2016-10-18
基金项目: 四川省科技支撑计划项目（15ZC1978）；四川省科研院所科技成果转化资金项目（14010139）
作者简介: 谢莹(1992-),女,陕西咸阳市人,硕士研究生,专业方向为药剂学。
参考文献
[1] 国家中医药管理局《中华本草》编委会. 中华本草[M]. 5版. 上海:上海科学技术出版社,1999:242.
[2] 王开祥,张慧,郑克岩,等. 白桦脂酸体外抗肿瘤的活性和机制[J]. 吉林大学学报,2009,47(3):622-627.
[3] 李万华,李琴,王小刚,等. 皂角刺中5个白桦脂酸型三萜抗HIV活性研究[J]. 西北大学学报,2007,37(3):40l-403.
[4] 武蕊娟,宋联强,谭镭,等. 马甲子的化学成分研究[J]. 中草药,2015,46(19):2834-2838.
[5] 赵玉丹,李双君,张桃,等. 马甲子果水提物中几类物质含量的测定和DPPHo活性的清除[J]. 广州化工,2015,43(23):156-158.
[6] 宋联强,武蕊娟,谭镭,等. HPLC测定马甲子药材中马甲子1#的含量[J]. 中国测试,2015,41(4):43-45.
[7] SUKSAMRARN A, TANACHATCHAIRATANA T, KANO-KMEDHAKUL S, et al. Antiplasmodial triterpenes from twigs of Gardenia saxatilis[J]. Journal of Ethnopharmacology,2003(88):275-277.
[8] GARCIA-CORTEZ D A, YOUNG M C M, MARSTON A, et al. Xanthones, triterpenes and a biphenyl from Kielm eyera coriacea[J]. Phytochemistry,1998,47(7):1367-1374.
[9] 周映霞,王振国,马莉,等. 白桦脂酸及其衍生物的研究概况[J]. 中国药学杂志,2005,40(6):405-407.
[10] 崔思娇,罗洁,张靓琦,等. UPLC同时测定酸枣仁中4种活性成分的含量[J]. 世界科学技术-中医药现代化,2013, 15(6):1323-1328.
