作者：李欣

作者单位：新疆医科大学 新疆地区高发疾病研究教育部重点实验室, 新疆 乌鲁木齐 830011

关键词：过氧化氢;荧光探针;生物检测

摘要：

过氧化氢（H₂O₂）是活性氧分子中重要的一种，人体正常水平的H₂O₂不会对机体造成损害。但是，细胞内过氧化氢的持续过度产生可能会导致细胞和生物分子的氧化损伤，引发生物体多种功能障碍，导致多种疾病的发生。因此，可视化、动态、实时地靶向和准确地定量细胞中的H₂O₂，对于了解其在细胞生理学中的作用进而用于病理诊断的标志物具有至关重要的作用。生物兼容性高的H₂O₂荧光探针可以对生物样本进行无损分析，具有广泛的应用前景。近年来，多种不同的H₂O₂荧光探针在相关生物学事件中的应用显著促进H₂O₂探针在医学和生物学领域的发展。该文介绍近年用于H₂O₂生物检测的有机探针及无机纳米探针两种荧光探针的研究机理及应用现状，并对探针的存在问题及研究方向进行展望，为进一步进行该领域的研究提供理论支撑。

Research progress of hydrogen peroxide fluorescence probes for biological detection
LI Xin
Key Laboratory of High Incidence Diseases Research in Xinjiang(Ministry of Education), Xinjiang Medical University, Urumqi 830011, China
Abstract: Hydrogen peroxide (H₂O₂), as an important molecule of reactive oxygen species, normal levels of which in the human body will not cause damage to the body. However, the continuous excessive production of hydrogen peroxide within cells may lead to oxidative damage to cells or biomolecules, various dysfunction of organisms, and the occurrence of many diseases. Therefore, visualization, dynamic, real-time targeting, and accurate quantification of H₂O₂ in cells are crucial for understanding its role in cell physiology and serving as biomarkers for pathological diagnosis. H₂O₂ fluorescent probes with high biocompatibility can perform non-destructive analysis of biological samples and have broad application prospects. In recent years, the application of various H₂O₂ fluorescent probes in related biological events has significantly promoted the development of H₂O₂ probes in the fields of medicine and biology. This article introduces the research mechanism and application advantages of organic probe and inorganic nanoprobe, used for H₂O₂ biological monitoring in recent years, and prospects the existing problems and research directions of the probes, providing theoretical support for further research in this field.
Keywords: hydrogen peroxide;fluorescence probe;biological detection
2023, 49(11):30-37  收稿日期: 2023-05-04;收到修改稿日期: 2023-06-15
基金项目: 新疆维吾尔自治区自然科学基金项目（2021D01C284）
作者简介: 李欣(1988-),女,新疆乌鲁木齐市人,高级实验师,研究方向为药物分析、生物医学工程学。
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